A simple, selective, rapid, precise and economical reverse phase high-pressure liquid chromatographic method has been developed for the simultaneous estimation of S (-) Amlodipine besylate and Nebivolol Hydrochloride for tablet formulations. The chromatographic separation was achieved on Hypercil column (250 × 4.6 mm, 5μ particle size) in isocratic mode with mobile phase consisting of Potassium dihydrogen phosphate buffer : acetonitrile : 0.1% triethylamine (60:40 v/v). The flow rate was 1ml / min and effluent was monitored at 269 nm PDA detection. The retention times of S (-) Amlodipine besylate and Nebivolol Hydrochloride were 7.55 ± 0.05 and 8.75 ± 0.05 minutes respectively. The linearity of the method was studied over the concentration range of 80-120 μg/ml for S (-) Amlodipine besylate and 80-120 μg/ml for Nebivolol Hydrochloride The limit of detection for S (-) Amlodipine besylate and Nebivolol Hydrochloride were found as 0.86μg/ml and 2.13μg/ml and and the limit of quantification for S (-) Amlodipine besylate and Nebivolol Hydrochloride were found as 2.6μg/ml and 6.4μg/ml respectively. The proposed method was applied for the quantitative determination of S (-) Amlodipine besylate and Nebivolol Hydrochloride in commercial combination formulations.
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